Hello all, I am performing PCR on about 1000 bacterial isolates (lysis solution only, no DNA extraction) for a culturomics project. PCR will amplify V1-V3 of 16S gene and then sending these out for sequencing to identify each isolate. I am searching for alternatives to running a small aliquot of each PCR product on a large...
Intro PCR is definitely one of those things in science that borders on magic and witchcraft. I like to think I know what I am doing, but I still have those days even in my postdoc that I have to pray to the PCR Gods to get something to work. In my experience, PCR success is mainly dependent on the primer set that you use....
Benchmarking CUT&Tag against ChIP-seq: https://www.biorxiv.org/content/10.1101/2022.03.30.486382v1 https://www.nature.com/articles/s41467-019-09982-5 Lovely blog post on CUT&Tag: https://www.activemotif.com/blog-cut-tag The improved sensitivity of CUT&Tag compared to ChIP-seq is due to the use of pA-Tn5 to streamline lib...
I have set up a very small lab for identification of human respiratory viruses for educational and research purposes. After some trial-and-error, the following is what I've found to be the simplest and most cost effective. It should go without saying that biosafety protocols and appropriate regulations must be followed. Fee...
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