You've prepared your epigenetic library for profiling histone marks. If you are not using the Zymo ChIP DNA Clean & Concentrator Kit for final elution, you may want to size select for your library region of interest using SPRI bead clean up. How much bead do you add relative to your volume of library? Because adapters for ...
The 10x multiome kit allows you to link open chromatin (ATAC) and gene expression at single cell resolution https://www.10xgenomics.com/products/single-cell-multiome-atac-plus-gene-expression?https%3A%2F%2Fwww.10xgenomics.com%2F%3Fsrc=search&utmmedium=search&utmsource=google&utmcampaign=sem-goog-2022-website-page-rag-pscmag...
Use the cellenONE https://www.cellenion.com/products/cellenone-x1/ if you'd like an open platform for the single cell -omics of your choice (e.g. I used it to develop new single cell epigenetic and multiomic profiling methods). It has: nanoliter dispensing so you can save on reagents temperature/dew point controlled inter...
Simultaneous Profiling of Gene Expression and Chromatin Accessibility in Single Cells ATAC-RNA Joint Profiling Workflow Though this could work for any type of gDNA library, e.g. CUT&Tag for profiling histone marks and TF binding) Sorting and DSP Fixation Sort 50,000-100,000 cells into RPMI + 10% FBS Spin down 300g 5 min...
Fresh out of my lab notebook, this is how I conducted RNA-seq with success from human/mouse FACS-purified cells. If you're a new user, you may ask NEB for free trial kits for all reagents listed in this protocol! Purify RNA using Monarch® Total RNA Miniprep Kit (T2010) https://international.neb.com/products/t2010-monarch-t...
In a genomics lab, if you need to work with really low input DNA samples you can use the Illumina Nextera XT kit. That being said, the kit is rather expensive and can be adapted to work perfectly fine by using 1/3 reagents for your reactions! You'll get pretty much the same results just make sure you keep the ratio of volum...
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