Hello fun(gi) friends! Did everyone see the two (!) papers published recently in Cell about the mycobiota profiles of tumors? One was from Rob Knight and Ravid Straussman's groups (https://www.cell.com/cell/pdf/S0092-8674(22)01127-8.pdf) and the other from Iliyan Iliev's group (https://www.cell.com/action/showPdf?pii=S0092-...
Hi everyone, I've received a custom oligo pool from Twist Bioscience and they provided 70ng of it. We talked to one of the reps and they said not to resuspend it to any less than 10ng/μL, which means I'll be adding less than 7μL of buffer, which is obviously not a lot. I was wondering if anyone had any practical tips for h...
Genetic EngineeringGeneticsMicrobiologyMolecular Biology Oligo Pool Twist Bioscience Twist Oligo Pools
First of all, @Michael Paulsmeyer had a great post a while back on how to get as much DNA from a standard miniprep as possible (https://www.scifind.net/methods/efficient-miniprep-spin-column-k). The variance in my personal results from minipreps is large, though. To make sure I always get a high concentration of DNA, I use ...
GOAL : To develop a safe and effective gene therapy vector! So excited and thankful to share these preliminary results. I am sharing this on youtube in more detail! https://youtu.be/twwOipz50WM In S/MAR vector the best promoter/terminator combination to use for gene expression is Ef1A/BGH in HepG2cells. As also demonst...
I compared the results from identical cloning protocols preceding either a heat-shock or electroporation bacterial transformation into NEB competent E. coli cells (chemically competent or electrocompetent) and found that the electroporation transformation consistently resulted in thousands more colonies than the heat-shock ...
In a genomics lab, if you need to work with really low input DNA samples you can use the Illumina Nextera XT kit. That being said, the kit is rather expensive and can be adapted to work perfectly fine by using 1/3 reagents for your reactions! You'll get pretty much the same results just make sure you keep the ratio of volum...
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