This is a method for low-cost electrotransformation of electrocompetent NEB turbo E coli cells using a homemade electroporation apparatus. The original paper upon which this method is based can be found here: https://doi.org/10.1371/journal.pbio.3000589 My method for preparing electrocompetent E coli can be found here: h...
Hi folks, I found a nice overview over the strength of various BioBricks promoters https://jbioleng.biomedcentral.com/articles/10.1186/1754-1611-3-4 I'm especially interested in finding a constitutive promoter (family) so I can test a range of promoters with different known strengths, for example the BioBricks J23100 prom...
Hi everyone, I've received a custom oligo pool from Twist Bioscience and they provided 70ng of it. We talked to one of the reps and they said not to resuspend it to any less than 10ng/μL, which means I'll be adding less than 7μL of buffer, which is obviously not a lot. I was wondering if anyone had any practical tips for h...
Genetic EngineeringGeneticsMicrobiologyMolecular Biology Oligo Pool Twist Bioscience Twist Oligo Pools
First of all, @Michael Paulsmeyer had a great post a while back on how to get as much DNA from a standard miniprep as possible (https://www.scifind.net/methods/efficient-miniprep-spin-column-k). The variance in my personal results from minipreps is large, though. To make sure I always get a high concentration of DNA, I use ...
I want to talk about preparing basic microbiological growth media and things that can go wrong when swapping reagents. Weird cloudy KB Agar A while ago, I was preparing KB Agar: Recipe for 1L KB Agar 10g Glycerol 20g Peptone 1.5g K2HPO4 trihydrate 12g Agar powder 5 mL sterile 30% MgSO4 solution (to be added after autocla...
Agar MediumAlcaligenes spp./Achromobacter spp.BacteriaGrowth Medium KB Agar Microbiology Ochrobactrum spp. Reagents Soil isolates Stenotrophomonas spp. Variovorax spp.
I compared the results from identical cloning protocols preceding either a heat-shock or electroporation bacterial transformation into NEB competent E. coli cells (chemically competent or electrocompetent) and found that the electroporation transformation consistently resulted in thousands more colonies than the heat-shock ...
I used this method to make electrocompetent NEB turbo E. coli cells. This protocol is based on the one from Barrick labs which is linked below. https://barricklab.org/twiki/bin/view/Lab/ProtocolsElectrocompetentCells One colony of NEB turbo E. coli was taken from a petri dish and inoculated into 5 mL of LB broth, in a 1...
CD BioSciences, a leading US-based service supplier focused on kinase/phosphatase biology, now introduces a series of structure characterization services for kinase/phosphatase biology research and drug development. CD BioSciences recruits a scientific team specializing in structural biology and bioinformatics, and offers s...
CD BioSciences, a leading customer-focused biotechnology company based in New York, introduces new GPCR purification services to support and facilitate GPCR research and drug development. CD BioSciences has built an unparalleled GPCR purification platform to provide the most reliable service for researchers. With more than...
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