Cell culture, antibodies and other reagents
U2OS, HeLa and RPE cells were obtained from ATCC and grown in Dulbecco’s modified Eagle’s media (DMEM) containing 10% fetal bovine serum, 2 mM L-glutamine, and penicillin (100 U/ml)/ streptomycin (100 μg/ml). Cas9-expressing K562 cells (Lu et al., 2018) were grown in RPMI 1640 Me...
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