Can I demultiplex sequenced data based on indexes on the forward primers if doing single end sequencing on HiSeq? ** ** I have prepared amplicon libraries using a different combination of forward and reverse primers. The forward and reverse primers used had different indexes, and I am planning to do 100bp single-end se...
Thank you very much for your recent protocol paper for CRISPR screening. I have couple of questions about the NGS primers for amplifying the sgRNA library. 1) We are planning to deep-sequence both mouse and human GECKO libraries (2-vector system) to check sgRNA representations in the same Miseq run via multiplexing each l...
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