I'm going to use gecko library in order to infect primary macrophages and is the very first time I'm approaching this technique, so I have some questions. I'm following the nat prot paper by Julia Joung (2017) but I have some questions regarding the titration part. I'm using the 2 vectors system and I will infect macropha...
I was hoping if someone could help me with a simple question. I really tried finding a clear answer to this online but I don't see a defined solution anywhere. This is my question: I am transfecting HEK293T cells with LentiCRISPRv2 + ssDNA via Lipofectamine 2000 in a 12 well plate. I added puromycin after 24 hours of selec...
I packed lentivirus from HEK293T cell line with lenticas9-blast #52962. I started selection with blasticidin 3 days post infection. Control group were dead and the infected cells were selected over one week. But it took at least one week to recover the cells (had to withdraw Blast) to have a decent number of cells to run WB...
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