Hello everyone I hope you are fine and doing great. I am a fresh graduate of Microbiology (Food and Nutrition). I want to hunt a postdoc in my research domain (Microbiome, Probiotics, Genomics). As per my research and collected information, the postdoc positions are competitive and the ones with a high number of pertinent ...
Twitter is my best friend and when I was goofing off researching the other day, I stumbled upon an interesting genomics paper. Now, I'm not the best in this field but even I can understand that there are certain aspects of our DNA that go entirely unnoticed, hence the purpose of the paper in question: > The clinical spect...
Intro PCR is definitely one of those things in science that borders on magic and witchcraft. I like to think I know what I am doing, but I still have those days even in my postdoc that I have to pray to the PCR Gods to get something to work. In my experience, PCR success is mainly dependent on the primer set that you use....
You've prepared your epigenetic library for profiling histone marks. If you are not using the Zymo ChIP DNA Clean & Concentrator Kit for final elution, you may want to size select for your library region of interest using SPRI bead clean up. How much bead do you add relative to your volume of library? Because adapters for ...
BAMBANKER Open to suggestions! This has worked incredibly well for me for both cell lines and FACS-purified cell populations from post-mortem human tissue. Will put in my manuscript reference as it becomes available. This has worked well for me for native ChIP-seq, CUT&Tag, and newer versions of CUT&Tag (to be updated by ...
Benchmarking CUT&Tag against ChIP-seq: https://www.biorxiv.org/content/10.1101/2022.03.30.486382v1 https://www.nature.com/articles/s41467-019-09982-5 Lovely blog post on CUT&Tag: https://www.activemotif.com/blog-cut-tag The improved sensitivity of CUT&Tag compared to ChIP-seq is due to the use of pA-Tn5 to streamline lib...
I have set up a very small lab for identification of human respiratory viruses for educational and research purposes. After some trial-and-error, the following is what I've found to be the simplest and most cost effective. It should go without saying that biosafety protocols and appropriate regulations must be followed. Fee...
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