This is a method for low-cost electrotransformation of electrocompetent NEB turbo E coli cells using a homemade electroporation apparatus. The original paper upon which this method is based can be found here: https://doi.org/10.1371/journal.pbio.3000589 My method for preparing electrocompetent E coli can be found here: h...
I compared the results from identical cloning protocols preceding either a heat-shock or electroporation bacterial transformation into NEB competent E. coli cells (chemically competent or electrocompetent) and found that the electroporation transformation consistently resulted in thousands more colonies than the heat-shock ...
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