11 months ago


Strains and Culture conditions V. cholerae strains used in this study are derived from E7946. Bacteria were routinely grown on LB agar plates and in LB broth with aeration at 37°C. Antibiotics were supplemented as appropriate at the following concentrations: 75 μg/ml kanamycin, 100 μg/ml spectinomycin, 1.25 or 2.5 μg/ml ch...
chimeric nuclease CRISPR/CASMolecular Biology
12 months ago

Materials & Methods

Bacteria and growth conditions Bacteria (Table S4) were cultured in lysogeny broth36 at 37°C. pCA24N-based plasmids37 were retained in overnight cultures via chloramphenicol (30 μg/mL), and kanamycin (50 μg/mL) was used for deletion mutants, where applicable. Spacer knockout To attempt to delete the CRISPR-Cas spacer reg...
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