This is a method for low-cost electrotransformation of electrocompetent NEB turbo E coli cells using a homemade electroporation apparatus.
The original paper upon which this method is based can be found here: https://doi.org/10.1371/journal.pbio.3000589
My method for preparing electrocompetent E coli can be found here: h...
Hi folks,
I found a nice overview over the strength of various BioBricks promoters https://jbioleng.biomedcentral.com/articles/10.1186/1754-1611-3-4
I'm especially interested in finding a constitutive promoter (family) so I can test a range of promoters with different known strengths, for example the BioBricks J23100 prom...
I found a used MJ Research PTC-100 Thermal Cycler for sale at $200, which is a really great price. However, it looks VERY old and the owner would not let me test it (he is a used equipment reseller so had zero experience with biology, and just wouldnt let me put some dubious liquid in his machine). The equipment powers on f...
I am working with carrots, which are, in general, very easy to culture. Just pop them on MS media with 2,4-D and they will produce callus within a month. My only problem is that my carrots seem to have a LOT of endophytic bacteria that take over very quickly. What can I do to suppress or kill these bacteria? I plan on using...
We know there's tons of troubleshooting information on Twitter so we've spoken with our community council and decided to start up a campaign to start archiving all sorts of experimental resolutions from across #sciencetwitter to add to our "lab-rary" using the tag #scifound. I've provided guidelines below to have an idea of...
Hello all, I am performing PCR on about 1000 bacterial isolates (lysis solution only, no DNA extraction) for a culturomics project. PCR will amplify V1-V3 of 16S gene and then sending these out for sequencing to identify each isolate. I am searching for alternatives to running a small aliquot of each PCR product on a large...
Hey all! I wanted to share this cool table from a new paper published yesterday in Nature from Hoberecht et al. that includes a useful list of many available sgRNA design websites/packages as well as their different functionalities (and lack thereof): https://www.nature.com/articles/s41467-022-34320-7/tables/1.
One of the ...
From Turing, Complexity to Programming of Biological Circuit Design.
Since the turn of the XXIth century when the term “digital biology” was coined circa 2005, there have been many revisionists that have sparked an interesting discussion of how information technologies and contemporary biology have merged and adapted into ...
Hi everyone,
I've received a custom oligo pool from Twist Bioscience and they provided 70ng of it. We talked to one of the reps and they said not to resuspend it to any less than 10ng/μL, which means I'll be adding less than 7μL of buffer, which is obviously not a lot. I was wondering if anyone had any practical tips for h...
First of all, @Michael Paulsmeyer had a great post a while back on how to get as much DNA from a standard miniprep as possible (https://www.scifind.net/methods/efficient-miniprep-spin-column-k). The variance in my personal results from minipreps is large, though. To make sure I always get a high concentration of DNA, I use ...

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