When doing cell adhesion studies, we want to know what percentage of the seeded cells actually adhered to the material (for example a thin, spin coated film on a coverslip). We can image the coverslip and using the area and cell density calculate this percentage. However, if the cells are not seeded uniformly, this can aff...
Dear Scientists, a while ago I made a video on how to count cells manually with counting chambers (e.g. Neubauer counting chamber). This video guide breaks down each step and explains how you can determine the number of cells in your solution with ease! Please check it out if you need to work with the Counting chambers an...
One way to determine the effect of treatments (e.g. drugs) on cell proliferation and survival is to work with the Colony Formation Assay. I have prepared a video guide on how to perform this assay: https://youtu.be/zPeY70vsHlk I seeded 1000 cells (HeLa) per well and incubated them with fresh medium after treatment (e.g. I...
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