I have experiences in RNAi and Cas9 knockout experiments in mammalian cells, but am now facing a little challenge. I have primary chicken and duck endothelial cells that require knockdowns of genes - knockouts of these genes are impossible due to their importance for these cells. I have seen 1-2 papers looking at CMV/Ef1a...
Good afternoon, I am a researcher from Brazil and I’m initiating a study with crispr cas13 focusing on diagnosis. I wanted to ask if you have any advice that can help a beginner. I’m open to suggestions, in a matter of sellers or distributors that you could recommend, like the best place to get this plasmid, the best reagen...
ne, I want to design a RNA detect system, and for Lwa Cas13a crRNA design, I suppose I need a sequence with DR sequence + crRNA ( from 5 ends to 3 ends). From the literuatures, I found some of the design employed 36 nt sequence as the DR sequence, and some employed the DR sequence with 16 nt, if there a principle for DR se...
everyone! Recently I designed four PspCas13b crRNAs at 5'UTR of KRAS, and made the KRAS crRNA pubulicated in Zhangfeng's paper as a positive control. When perform qPCR to detect the expression level of gene, only the positive control worked but four crRNAs. So I want to know whether the design principle of crRNA shoul...
Find your community. Ask questions. Science is better when we troubleshoot together.
Sci Find is a place for experimentation beyond the publication. We are a growing collaborative community of scientists and experimentalists from around the world who believe science is better when we work together.
- Help us keep Sci Find a safe space for productive and collaborative conversations. Please be kind and respect your fellow community members. If you see abusive or alarming content, please report it to firstname.lastname@example.org, and our team will take appropriate actions.
- All information posted to this page is public and Open Access. Learn more about our commitment to Open Access by visiting our Docs.
- We encourage our community to have collaborative troubleshooting discussions, and to share tips and tricks, experimental methods, resources, and opportunities.
- You must be a member to create or engage with content. Sign Up to create your profile and join the community for free.
- Follow a guild and create a post. Learn more about guilds here.
- Post titles should be descriptive and include descriptive tags. Check out our tagging guidelines here.
Have a question?
Contact email@example.com or check out our support page.