I have experiences in RNAi and Cas9 knockout experiments in mammalian cells, but am now facing a little challenge.
I have primary chicken and duck endothelial cells that require knockdowns of genes - knockouts of these genes are impossible due to their importance for these cells.
I have seen 1-2 papers looking at CMV/Ef1a...
Do you have any recommendations in terms of tools for guide RNA design for cas13? And does the crRNA of active cas13 works for deactivated cas13d.
Good afternoon, I am a researcher from Brazil and I’m initiating a study with crispr cas13 focusing on diagnosis. I wanted to ask if you have any advice that can help a beginner. I’m open to suggestions, in a matter of sellers or distributors that you could recommend, like the best place to get this plasmid, the best reagen...
ne, I want to design a RNA detect system, and for Lwa Cas13a crRNA design, I suppose I need a sequence with DR sequence + crRNA ( from 5 ends to 3 ends). From the literuatures, I found some of the design employed 36 nt sequence as the DR sequence, and some employed the DR sequence with 16 nt, if there a principle for DR se...
everyone!
Recently I designed four PspCas13b crRNAs at 5'UTR of KRAS, and made the KRAS crRNA pubulicated in Zhangfeng's paper as a positive control. When perform qPCR to detect the expression level of gene, only the positive control worked but four crRNAs. So I want to know whether the design principle of crRNA shoul...
I am interested in targeting mRNA with Cas 13. Is there an online tool to design gRNA for Cas13?
I was wondering if anyone has successfully used the cas13 (LwaCas13a, PspCas13b, or RfxCas13d) system in mammalian cells for gene know down. What are your experiences with these systems?
let me know.

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