I know there are some softwares that exist that can help determine if a cell is a macrophage, neutrophil, RBC, etc. but those usually cost a lot of money and are tissue sample specific. Does anyone know of any other programs (low cost or free) that can determine what cells are in Bronchoalveolar lavage (BAL) fluid after H&E...
Christophe Langouet-Astrie
Graduate Student
University of Colorado Denver, Anschutz
Aurora, CO
Eureka Score 2
Intro
My interests are to help improve critical care patient outcomes and responses by understanding infectious lung injury and their risk for secondary bacterial infections that can lead to additional lung injury, systemic responses, and poor patient outcomes. I additionally investigate how glycocalyx, composed of glycosaminoglycans, can influence these host (healthy or injured) pathogen interactions.
Disciplines
Biotechnology Human Biology Molecular Biology
Skills & Expertise
Bioinformatics Lung Biology Pneumonia Sepsis Glycobiology Glycosaminoglycans
No certifications added.
Models & Organisms
Mouse Staphylococcus aureus Influenza virus Human
No instrumentation added.
No posts added.
Methods
Does anybody have any insight/protocol optimizations on western transfers ( I have an iBlot2 but can do it the old fashion way too). When staining the gel post-transfer it looks that 80-90% of the protein remains on the gel with minimal to no protein is on the membrane. Looking to identify proteins in the 30-60kDa range and...
Publications
Experience
Graduate Research Assistant
University of Colorado Denver, AnschutzFull Time
August 2018 - Present
Aurora, CO United States
Studying host pathogen interactions in primary and secondary (superinfection) pneumonias
Hematology Researcher
University of Colorado Denver, AnschutzFull Time
October 2016 - June 2018
Aurora, CO United States
Research focus was on personalized medicine for patients with hematological malignancies. Complementing statistical and bioinformatics approaches with molecular biology lab techniques, I investigated what determines myeloma and lymphoma patient sensitivities to commonly used and investigational agents. In doing so, my goal was to help discover new biomarkers that may guide therapy decisions. Techniques included large data set bioinformatics, statistical programming, CRISPR gene editing, patient sample analyses, flow cytometry.
Undergraduate Researcher
James Madison UniversityPart time
January 2012 - May 2015
Harrisonburg, VA United States
Have worked on isolating and characterizing bacteriophages that are specific to Bacillus thuringiensis one of which has been published, Waukesha92. The phage was sequenced, annotated, compared to other phages on Genbank, and looked at host range infectivity. During fall semester 2012, a group of undergraduates were isolating bacteriophage from water samples in order to find the MecA resistence gene which could determine one mode of genetic transfer in methicillin resistant Staphylococcus aureus (MRSA). This work was with collaboration with VCU Starting in the fall of 2013, looked for alternative treatments of fireblight, Erwinia amylovora, that infects pear and apple trees. Have isolated one strain of Erwinia from pear and apple orchards and have isolated potential novel antibiotics.
Education
University of Colorado Denver, Anschutz Medical Campus
PhD
Pharmacology
2018 - 2023
Focus in pulmonary sciences and critical care
University of Colorado Denver, Anschutz Medical Campus
MS
Biomedical Sciences and Biotechnology
2016 - 2018
James Madison University
BS
Biotechnology
2011 - 2015
Statistics Minor