Troubleshooting Gecko Sequencing on HiSeq 4000

Has anyone used a HiSeq 4000 to sequence Gecko libraries? What % PhiX did you use? -we will have 6 different barcodes. Any other tips? or things to consider?


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Fuchsia Kelpiealmost 5 years ago

I would recommend using 10 different forward primers, each with different staggered bases so you will not need to spike in as much PhiX. The details are described in recent Nat Protoc paper.

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