today the question comes up at which timepoints NGS of the library transduced cells are recommended, during the screening experiment.
Normaly i would say,
1. sequencing the amplified libarary plasmid.
2. after transduction and 7 days of puromycin Selection = d0 Start of screening experiment
3. after finishing your screen with the substance of choice =dx end of experiment.
The question is, would it make sense to sequence also after transduction and before selection with Puromycin?