sgRNA scaffold for CjCas9

I would like to use a Cas9 from C. jejuni, however I couldn't find any gRNA expression vector for it on Addgene as I understand it requires its very own scaffold and cannot be used with SpCas9 gRNA scaffold.

Are you planning to add such plasmid to Addgene or can you suggest an alternative?


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Bronze Impabout 4 years ago

This plasmid from Addgene expresses Cj gRNA from U6 promoter https://www.addgene.org/89753/

Crimson Hippogriffabout 4 years ago

Perfect!

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