Sequencing mislabeled cell lines

I mislabeled some cell lines that I transduced with lentiguide puro. Does anyone have some proven primers that work to pcr and sanger sequence the guides?


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Amethyst Werewolfover 2 years ago

A few questions.
1 is this ko or insertion
2 how many guides do you have and where are they located on the dna
3 how were you going to check it would cut.

Sienna Ghoulover 2 years ago

Its a base editing experiment. There are six guides total. I plan on using sanger sequencing to get a readout on efficiency, but these primers dont help as they are in the genomic locus. What im looking for are primers within the integrated lentiguide puro that can be used to figure out which cell line got which guide. Of course it is easy enough to design, but i figured if there are standard, proven primers for this purpose, id be better off using these.

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