I've made a CRISPR KO cell line using the px459 vector. I would now like to perform a rescue experiment where I stably re-express the gene in the KO cell line by using for instance a plasmid encoding the gene cDNA? I would like to confirm that the CAS9 and sgRNA from px459 are not integrated in the genome of the cells and therefor should not cleave any plasmid encoding for the gene. Tlhank you.
Login or Signup to leave a comment
Find your community. Ask questions. Science is better when we troubleshoot together.
Have a question?
Contact email@example.com or check out our support page.