I have expanded the gecko library and submitted for NGS to check for the representation.
I currently have a skew ratio of 10.9 and 10.7 for libraries A and B, respectively.
Would you recommend re-expanding the libraries given the skew ratio is >10 or do you think it is close enough to proceed?
|Title||Library A||Library B|
|Percent of Reads with sgRNA||86%||87%|
|Percent of reads with perfect sgRNA match||79%||79%|
|Number of unique sgRNA||63950||56869|
|Percent of detected sgRNAs||100%||100%|
|Percent of undetected sgRNAs||0.01%||0.07%|