Re: no mutation in mutant

Do any of your CRISPR treated samples show mutagenesis?

Do you have plants regenerated from a control treatment (selection without sgRNA etc.)? Maybe some white plants might emerge from somoclonal variation?

Maybe your gRNA favors larger deletions that you cannot pick up with a small PCR product assay, so you are only observing wild type alleles when most copies may be knocked out resulting in the white phenotype.


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Ruby Wendigoover 3 years ago

Somaclonal variation (if your plants are going through tissue culture) is the most likely reason you observe albino phenotype but no edit in the PDS.

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