Positive screening and no hits with low FDR

We performed a positive screening with GECKO v2 full library and after screening and NGS, we could see none of the genes had a positive ranking with lower FDR. With only one Gene as a top hit, with lower FDR which we think as a result of positive screen selection.
Please suggest some inputs regarding this issue.
Thanks in advance.


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Fuchsia Kelpieover 2 years ago

I would recommend trying to validate the top 3 genes to see if your screen worked at all first. If the top 3 genes do indeed validate, then you can try to reduce the background noise in your screen by applying a more stringent selection pressure.

Bronze Basiliskover 2 years ago

Thanks for your kind reply. We have already applied a more stringent selection pressure and thats why we hardly got any hits. We are trying to repeat the screening with less stringent pressure which might able to give us some additional hits.

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