Dear CRISPR lovers!!
I have been working with CRISPR/Cas9 and I must to evaluate the Off-Target effects of my system. However, the current strategies (NGS) are so expensive. Macrogen has the service but each sample is around USD 760 and I want to test 20 samples (10 from UT samples and 10 from transfected cells).
Anyone knows suppliers less expensive? Do you think that using Sanger sequencing of sorted cells could be enough?
Thanks in advance,