Multiple sgRNAs with ribozyme sequences

Are there papers out there showing delivery of multiple sgRNAs from one RNA, by using multiple self cleaving ribozyme sequences (HH/HDV?) are there known limitations next to repetitiveness which could complicate DNA synthesis/cloning?


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Lemon Lamiaalmost 3 years ago

this paper describes the use of ribozymes to self-cleave gRNA arrays: doi: 10.1093/nar/gkw1048

It is from 2017, so there might be newer papers out there that cite this one.

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