lncRNA KO mouse model

Does anybody have good experience in KO lncRNA using electroporation of mouse zygotes?

I would very much like to learn from experience re strategy please.


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Turquoise Garudaabout 3 years ago

We do routinely KO mice with CRISPR electroporation. We follow Chen et.al. 2016 protocol and it works with great efficiency for us (last project 100% of F0 pups has KO in the targeted locus).

White Elfabout 3 years ago

May I ask if you also use the same electroporation device (Bio-Rad Gene Pulser) from the Chen et.al. 2016? Does the CRISPR Knock-in also work using the CRISPR-EZ method in your experience?

Purple Dragonabout 3 years ago

Thank you!
I ask specifically for long-non-coding RNA KOs.

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