lentiSAMv2 activation without lentiMPH v2?

Can lentiSAMv2 (with a guide RNA) activate without using lentiMPH v2? I recall that lentiMPH v2 just improves the efficiency of activation for some genes.


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Fuchsia Kelpieabout 4 years ago

It really depends on your gene and cell type and the level of activation you need to get your phenotype. Using lentiSAMv2 with lentiMPHv2 is a safer bet because there are a lot of cases where using only lentiSAMv2 (especially in a protein-limited, lentiviral setting) will not significantly activate your gene of interest. I would recommend experimentally testing this out for your particular gene and cell type.

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