knockout in OVCAR3

Did anyone make Crispr-Cas9 guiding Knockout in OVCAR3 cell line? We used gRNA which worked successfully in other cell lines to do transient transfection in OVCAR3 but cells can not stand puromycin selection after transfection. We now think to switch to a GFP backbone to avoid that. But wonder if anyone have similar experience.


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Azure Krakenalmost 5 years ago

Have you considered an RNP approach? You can get three free synthetic modified sgRNAs for use with Cas9 (or a free Gene Knock Out (GKO) kit- which contains everything you need for RNP formation and positive controls). The only downsides are 1) you generally have the

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