How to design a CRISPR experiment ?

for a seminar at university, I was asked to plan a CRISPR experiment, focusing on methods and protocols.

Can someone please refer me to an article describing such experiment? I don't need a specific topic, I'm interested in the method used.


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Seashell Griffinalmost 3 years ago

Pohl C., Mózsik L., Driessen A.J.M., Bovenberg R.A.L., Nygård Y. (2018) Genome Editing in Penicillium chrysogenum Using Cas9 Ribonucleoprotein Particles. In: Braman J. (eds) Synthetic Biology. Methods in Molecular Biology, vol 1772. Humana Press, New York, NY

Wine Chimeraalmost 3 years ago

This protocol, comprised into a magnificent book chapter, is very helpful to work with in vitro filamentous fungi genome editing, and in some case could be adapted to work with higher fungi.

I recommend this for all researcher working with genome edition of fungi!

Amethyst Sirenalmost 3 years ago

I would like to experiment using CRISPR is it possible to use it in grass seeds? I am looking to make grass glow

Seashell Griffinalmost 3 years ago

Not sure about the common DNA delivery method for the organisms. You can easily incorporate this method into your existing PEG mediated transformation.

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