I am planing to design and generate a small pooled CRISPRi/a library with around 50-80 genes (3-5 gRNAs per gene) that enable screens with single-cell RNA-seq readouts. I am wondering does anyone have any idea how many non-targeting gRNAs as negative controls should I include in the library? Is it necessary to also include any positive controls in the library. Thank you so much.
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