Login or Signup to leave a comment
Pink Pixiealmost 4 years ago

The "chimeric guide RNA" was the name given to the very first version of sgRNAs (http://science.sciencemag.org/content/337/6096/816.long and http://science.sciencemag.org/content/339/6121/819.long). It was kind of a bare bones version of a sgRNA-- it only had the absolutely essential components of the original direct repeat and tracrRNA necessary for editing DNA in a test tube, but nothing more.

George Church's lab then called them "guide RNAs" (http://science.sciencemag.org/content/339/6121/823.long) and Keith Joung's lab adopted the name single guide RNAs (sgRNAs) in the first zebrafish editing paper-- https://www.nature.com/articles/nbt.2501.

Eventually the name "sgRNAs" stuck- although the sgRNAs we use now have a couple modifications relative to the original "chimeric guide RNA" used 6-7 years ago (elongated tracrRNA element and in some cases a mutated polyT track and extended hairpin loop).

Find your community. Ask questions. Science is better when we troubleshoot together.
Find your community. Ask questions. Science is better when we troubleshoot together.

Have a question?

Contact support@scifind.net or check out our support page.