Green fluorescence gene

How could I use GFP as an indicator of transfection efficiency of Px330 (CRISPR) vector in to cell line?

Is it limited to cell lines that already express GFP gene?


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Brown Valkyriealmost 4 years ago

You use another commercial vector that carry GFP as transfection control and transfect it in another well concurrently with transfecting your CRISPR vector.

But for CRISPR, I would recommend transduction rather than transfection!

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