GeCKO version2 Human Library sgRNA annotation files

Does anyone have GeCKO version2 human library sgRNA annotation files (one vector)? I need positions of all sgRNA for my experiments. Thank you very much.


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Peach Dragonover 3 years ago

Hi

You can find all data in Addgene website:

https://www.addgene.org/pooled-library/zhang-human-gecko-v2/

You can also find some more data in Shalem, 2014 Science in the supp file:

https://science.sciencemag.org/content/sci/suppl/2013/12/12/science.1247005.DC1/Shalem.SM.pdf

(sequences and positions in the plasmid).

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