Donor arm size for a 2 kb insert

I want to knock in an insert by CRISPR. The insert is 2kb. For the arm size on each side, is 800bp enough? Or should I make it longer?


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Green Bansheeover 4 years ago

It's enough.

Burgundy Cerberusalmost 3 years ago

I want to do a similar size insertion. What plasmid/system did you end up using?

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