I am using Gecko v2 library to screen survival cells after drug treatments.
After drug treatments, I have harvested cells and count the cell numbers.
As expected, I have 4 times more numbers of cells in the control group (untreated) than in tested groups. I expect that I have more genomic DNA in the control group too.
So I am wondering if I have to use up the genomic DNA from the control group for the PCR prior to NGS or if I need to use the same amount of genomic DNA from each group.