differences between control and test groups prior to PCR for NGS

I am using Gecko v2 library to screen survival cells after drug treatments.

After drug treatments, I have harvested cells and count the cell numbers.

As expected, I have 4 times more numbers of cells in the control group (untreated) than in tested groups. I expect that I have more genomic DNA in the control group too.

So I am wondering if I have to use up the genomic DNA from the control group for the PCR prior to NGS or if I need to use the same amount of genomic DNA from each group.


Login or Signup to leave a comment
Fuchsia Kelpieover 2 years ago

This depends on how many cells you have in each group. From each group, you should use up all of the genomic DNA from enough cells to maintain screening coverage, or 500 cells/guide RNA in your screen.

Pearl Mummyover 2 years ago

Thank you very much.

Find your community. Ask questions. Science is better when we troubleshoot together.
Find your community. Ask questions. Science is better when we troubleshoot together.

Have a question?

Contact support@scifind.net or check out our support page.