I'm trying to perform a drug screening and i know how to do it for positive selection (NGS before and after drug with a 500X coverage according to the last paper from 2017). But i'm also interested in the negative selection part of the screening and here is where i have doubts:
-I need to perform NGS in more time points (ex. before-during-after treatment) or just before-after is ok?
-The NGS parameters are the same?coverage, amount of gDNA to analyse?