designing gRNA

I'm wanting to design gRNA for CRISPR/Cpf1 (AsCpf1), is it possible to design gRNA for intergenic spacer, a subset of noncoding DNA?


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Fuchsia Kelpieover 4 years ago

Yes it is - as long as you can find a PAM, which should be easier for Cpf1 in noncoding regions that are more AT rich.

Gray Wyvernover 4 years ago

Most of the time, we choose two gRNAs to delete the noncoding DNA, which the frame-shift may be not work.

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