crispri gRNAs selection

I am starting to work with CRISPRi and wonder what could be the golden rule for gRNAs selection?

which area will be the best from TSS?100-500bp upstream?

any favorite sites for CRAB?

Is there any inducible dCas9-KRAB-gRNA plasmids available?


Login or Signup to leave a comment
Fuchsia Kelpieover 4 years ago

For dCas9-KRAB, I usually target 0-200bp downstream of the TSS. The published sgRNAs for genes like B4GALNT1 all work relatively well. You can check Addgene to see if there are any inducible CRISPRi plasmids available.

Ivory Griffinover 4 years ago

I'm working with consitutive system and I put my Sg RNAs upstream or downstream of the TSS but everytime at +/- 300bp of the TSS.
You have to try different guides for each target and select the most efficient.

Find your community. Ask questions. Science is better when we troubleshoot together.
Find your community. Ask questions. Science is better when we troubleshoot together.

Have a question?

Contact support@scifind.net or check out our support page.