CRISPR plasmid transfection

Does the design of gRNAs that target GFP gene ,an indicator of transfection efficiency, as well as gene of interest is limited to cell line that already express GFP?


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Brown Valkyriealmost 4 years ago

No, they should be an indicator of CRISPR/Cas9 cutting effieciency, in other words, that your cell express both Cas9 protien and gRNA in a functional way.

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