I am trying to make a Caco-2 Cas9 cell line by lentiviral delivery (pLentiBlast and Hygro) but I have been having issues with it.
I just wanted to ask if any has made this cell line or manipulated Caco-2s with CRISPRs, to know if it might be particularly resistant or maybe I just need to workout the protocol for it.
Many thanks in advance !!!