We recently collected genomic DNA from a screen. We did PCR using about 3-5ug of DNA per well on a 96-well plate and got 5ml of PCR product to purify. So what to do next to purify the 300bp PCR band for Hi-seq? __ __
Do you do PCR purification followed by gel separation? I was wondering whether the large amount of genomic DNA (300-500ug) in the PCR mixture will overload the Qiagen columns, which has a binding capacity of 10ug. Or do you use ethanol/sodium acetate to precipitate both genomic DNA and PCR products, and then do gel separation?
Any advise would be much appreciated! __ __