How to Re-use Agilent Bioanalyzer 2100 DNA Chips in the lab!

Since working in a genomics lab, one of the nifty tricks I learned was how to re-use the Agilent Bioanalyzer 2100 DNA chips (Cat No.5067-4626) The chips are the limiting reagent and you can get quite a bit of use out of your gel by doing this method. It also means, in a pinch, if you are running out of chips or reagents that you can always have some around to run the bioanalyzer. It’s a pretty simple process but has saved me a bunch of times (and countless dollars). I’ve used it for running gDNA (diluted of course), and libraries.

You can follow the protocol from this paper that outlines it and download it from their supplementary materials (https://www.future-science.com/doi/full/10.2144/000114232). The protocol is perfect and I’ve run it as is with no problems. Some of the chips I’ve even used up to 20 times without fail. I’ve tried it with RNA chips before too but it’s quite finicky what with the easily degradable nature of RNA. I never did protein work so not sure how it works in that realm.


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Stefani Robnettover 1 year ago

This looks really helpful!

Christopher White4 months ago

This looks interesting.

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