As a PLATFORM for SYSTEMS BIOLOGY, the open-source project LIFE123 (https://life123.science) offers a growing set of tools to wrestle with the complications of numerical solutions to complex dynamical systems, such as cycles of biochemical reactions. ONLY 1/2 OF THE BATTLE, is to offer functions to set up and simulate webs...
Did you know: Altos Labs raised $3 billion in series A in 1/2022! 2022 LONGEVITY FUNDING Deals by Type. Notice the preeminence of Series A and Seed. For more fascinating info: https://spannr.com/reports/2022-longevity-funding Want to get more involved? Check out the Alliance for Longevity Initiatives (A4LI), a non-pro...
This is a method for low-cost electrotransformation of electrocompetent NEB turbo E coli cells using a homemade electroporation apparatus. The original paper upon which this method is based can be found here: https://doi.org/10.1371/journal.pbio.3000589 My method for preparing electrocompetent E coli can be found here: h...
CYCLES of reactions are the bread-and-butter of biological networks... Explore them with just a few lines of Python code! (optionally in a 1-click hosted environment 😀 ) Pleased to announce version Beta 21 of the COLLABORATIVE OPEN-SOURCE project LIFE123 (https://life123.science) : Computational Systems Biology, with em...
Hi folks, I found a nice overview over the strength of various BioBricks promoters https://jbioleng.biomedcentral.com/articles/10.1186/1754-1611-3-4 I'm especially interested in finding a constitutive promoter (family) so I can test a range of promoters with different known strengths, for example the BioBricks J23100 prom...
I am working with carrots, which are, in general, very easy to culture. Just pop them on MS media with 2,4-D and they will produce callus within a month. My only problem is that my carrots seem to have a LOT of endophytic bacteria that take over very quickly. What can I do to suppress or kill these bacteria? I plan on using...
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